S R Group

This assay uses the quantitative enzyme immunoassay method. The provided microtiter plate in the kit is pre-coated with GFAP(Glial Fibrillary Acidic Protein). Standards or samples are added to the appropriate wells of the microtiter plate, along with a biotin-conjugated antibody specific to GFAP(Glial Fibrillary Acidic Protein). Then, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Following this, TMB substrate solution is added. Only the wells that contain GFAP(Glial Fibrillary Acidic Protein), biotin-conjugated antibody, and enzyme-conjugated Avidin will show a color change. The enzyme-substrate reaction is stopped by adding a sulfuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GFAP(Glial Fibrillary Acidic Protein) in the samples is determined by comparing the optical density (OD) of the samples to the standard curve.

This assay uses the quantitative enzyme immunoassay method. The provided microtiter plate in the kit is pre-coated with IL1a(Interleukin 1 Alpha). Standards or samples are added to the appropriate wells of the microtiter plate, along with a biotin-conjugated antibody specific to IL1a(Interleukin 1 Alpha). Then, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Following this, TMB substrate solution is added. Only the wells that contain IL1a(Interleukin 1 Alpha), biotin-conjugated antibody, and enzyme-conjugated Avidin will show a color change. The enzyme-substrate reaction is stopped by adding a sulfuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IL1a(Interleukin 1 Alpha) in the samples is determined by comparing the optical density (OD) of the samples to the standard curve.

This ELISA kit uses quantitative-ELISA as the method. The Micro-elisa strip plate provided in this kit has been pre-coated with an antibody specific to catecholamine. Standards or samples are added to the appropriate Micro-elisa strip plate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for catecholamine is added to each Micro-elisa strip plate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain catecholamine and HRP conjugated catecholamine antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of catecholamine. You can calculate the concentration of catecholamine in the samples by comparing the OD of the samples to the standard curve.

This assay uses the quantitative enzyme immunoassay method. The provided microtiter plate in the kit is pre-coated with IL5(Interleukin 5). Standards or samples are added to the appropriate wells of the microtiter plate, along with a biotin-conjugated antibody specific to IL5(Interleukin 5). Then, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Following this, TMB substrate solution is added. Only the wells that contain IL5(Interleukin 5), biotin-conjugated antibody, and enzyme-conjugated Avidin will show a color change. The enzyme-substrate reaction is stopped by adding a sulfuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IL5(Interleukin 5) in the samples is determined by comparing the optical density (OD) of the samples to the standard curve.