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Addition Information :
The Blood DNA Isolation Kit by Qiagen is an essential tool for researchers and laboratories seeking to obtain high-quality DNA from blood samples with efficiency and reliability. This kit employs a streamlined protocol that minimizes the time required for DNA extraction while maximizing yield and purity. Each component is meticulously designed to ensure optimal lysis of blood cells, followed by effective binding of DNA to the provided silica membrane, which facilitates the removal of contaminants and inhibitors. The result is a clean, concentrated DNA solution that is ideal for downstream applications such as PCR, sequencing, and genotyping. With its user-friendly instructions and robust performance, the Qiagen Blood DNA Isolation Kit is the perfect choice for any laboratory aiming to achieve consistent and reproducible results in their genetic analyses.

In this kit. Combining the method of preparing membrane with high adsorption amount of DNA and selectively adsorbing DNA to achieve the purpose of rapid purification of plasmid DNA. It applies to extract up to 60ug of high purity plasmid DNA from 5-30 ml bacterial culture. It has been used in molecular biology experiments such as sequencing. in vitro transcription and translation. restriction endonuclease digestion and bacterial transformation.

The kit is suitable for extracting high purity total DNA from fresh or frozen animal tissues, cells, blood, bacteria and other samples. DNA fragments with a maximum molecular weight of 50 KB can be purified without the use of toxic solvents such as phenol or chloroform and ethanol precipitation. The optimized buffer system was used to efficiently and specifically bind DNA from the pyrolysis solution to the silica-based centrifugal adsorption column. PCR and other inhibitors of enzymatic reaction could be effectively removed by two-step washing steps. Finally. high purity DNA could be obtained by using low-salt buffer or water elution. The purified DNA can be directly used in enzyme digestion, PCR, Real-Time PCR, library construction, Southern Blot, molecular markers and other downstream experiments.

This kit uses a unique buffer system that combines with traditional isopropanol precipitation to quickly obtain large amounts of high-purity plasmid DNA. The plasmid DNA extracted using this kit can be applied to various routine operations. Including restriction enzyme digestion, PCR, sequencing, ligation, transformation and transfection of a variety of cell experiments.It is recommended to use the amount of bacteria every time: the recommended amount of high-copy plasmid is 100 ml. the yield is generally about 200-1500ug the recommended amount of low-copy plasmid is 200 ml. and the yield is generally about 100-400ug.

When the width of the sample well during electrophoresis is less than 5 mm, a clear band can be obtained by taking 5 μl Marker electrophoresis each time. If the sample hole is widened, the sampler volume of Marker must be increased appropriately.**2. For DNA electrophoresis, the purity of Agarose greatly affects the clarity of DNA bands. Therefore, the best quality Agarose should be used for electrophoresis. The recommended gel concentration is 0.8% to 1%.**3. When performing Agarose electrophoresis, the concentration of Agarose is closely related to the separation performance of DNA fragments. The greater the concentration of Agarose, the better the separation performance of short DNA; conversely, the smaller the concentration of Agarose, the more favorable for the separation of long DNA.*

DNA Marker IV is composed of 7 kinds of DNA fragments with a length of 200 bp to 4,500 bp, dissolved in 1×Loading Buffer, and can be used for direct electrophoresis of 5-10 μl during use, which is very convenient to use

Host: Rat
Product: IgG, Fab fragment
Conjugate: Unconjugated
Product Category: ChromPure® Purified Proteins from Normal Serums
RRID: AB_2337138

Host: Human
Product: IgG, Fab fragment
Conjugate: Alexa Fluor® 488
Product Category: ChromPure® Purified Proteins from Normal Serums
RRID: AB_2337103

Host: Human
Product: IgG, Fab fragment
Conjugate: Cyanine Cy™3
Product Category: ChromPure® Purified Proteins from Normal Serums
RRID: AB_2337078

This kit can recover high-purity DNA fragments containing no salt or low salt concentration and no impurities such as protein and RNA from various concentrations of agarose gel. The 200 bp-10 kb DNA fragment has a recovery rate of 80% or more. and single-stranded. double-stranded DNA fragments and circular plasmid DNA can be recovered. The recovered fragment DNA can be directly subjected to enzyme digestion,enzyme ligation,and sequencing reaction.

TRIpure Total RNA Extraction Reagent is a reagent for total RNA extraction from various animal and plant, bacterial tissues/cells. It has strong cleavage ability to lyse cell and tissue samples in a short time and keep RNA intact in the sample. and effectively inhibit the degradation of RNA. The sample can be fully cleaved in the reagent. After centrifugation with chloroform, the solution forms a supernatant layer, an intermediate layer and an organic layer (lower red layer). The RNA is distributed in the upper aqueous phase, and the supernatant layer is collected. Total RNA can be recovered by precipitation of propanol. The extracted total RNA is high in purity and contains no protein or genomic DNA. It can be directly used for Northern, dot hybridization, mRNA purification, in vitro translation, RT-PCR, poly(A)+ selection, RNase protection analysis, and construction of cDNA libraries. A variety of molecular biology experiments.

The reagents used in this kit do not contain phenol and chloroform, which greatly reduces the harm of phenol and chloroform to the experimenter and expands the use environment. This product can quickly extract total RNA from animal cells, tissues, and can process a large number of different samples simultaneously. Extracted total RNA with high purity and extremely low contamination of proteins and other impurities, which can be used for RT-PCR, Real Time RT-PCR, chip analysis, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis and molecular cloning Various downstream experiments.

A 3-Color Prestained Protein Marker (10-190 kDa) is a ready-to-use mixture of 10 proteins, covalently linked to blue, green, and orange dyes, designed for SDS-PAGE and Western blotting, allowing easy visualization of protein separation, accurate size estimation (10-190 kDa range), and confirmation of transfer efficiency, featuring bright, distinct bands for clear tracking without heating or dilution. 

Host: Human
Product: IgG, Fc fragment
Conjugate: Horseradish Peroxidase
Product Category: ChromPure® Purified Proteins from Normal Serums
RRID: AB_2337055

Host: Human
Product: IgG, Fab fragment
Conjugate: Fluorescein (FITC)
Product Category: ChromPure® Purified Proteins from Normal Serums
RRID: AB_2337070

Host: Chicken
Product: IgY (IgG), whole molecule
Conjugate: Alexa Fluor® 594
Product Category: ChromPure® Purified Proteins from Normal Serums
RRID: AB_2336977

A 3-Color Prestained Protein Marker (2.7-40 kDa) is a ready-to-use solution of nine different proteins, dyed in blue, green (at 10 kDa), and orange (at 40 kDa)

Ampicillin (AMP) 10 µg refers to small, filter paper discs containing 10 micrograms of the antibiotic ampicillin, used in microbiology labs for the Kirby-Bauer method (disc diffusion test) to determine if a specific bacteria is sensitive or resistant to ampicillin, guiding clinical treatment for infections like meningitis, pneumonia, or UTIs. These discs help identify effective treatments by measuring zones of bacterial growth inhibition on agar plates, with "AMP 10" printed for easy identification. 

Bacitracin B 10 IU refers to a standard concentration of the antibiotic bacitracin, typically found in small paper discs used in microbiology labs for Antimicrobial Susceptibility Testing (AST), especially the Kirby-Bauer method to check if bacteria like Streptococcus pyogenes are sensitive to it, helping guide treatment decisions. These discs contain 10 International Units (IU) of bacitracin and help determine effective antibiotic regimens for infections. 

Plasmocure™ is a second-line anti-mycoplasma reagent. It is recommended for the elimination of mycoplasma that appear to be Plasmocin™ -resistant 

Imiquimod (R837), an imidazoquinoline amine analog to guanosine, is an immune response modifier with potent indirect antiviral activity. It is an FDA-approved treatment for external genital warts, basal cell carcinoma, and bladder cancer. Imiquimod is a specific agonist for human and mouse Toll-like receptor 7 (TLR7). TLR7 and TLR8 are endosomal pattern recognition receptors that play an important role in the antiviral immune response [1]. 

Host: Rat
Product: IgG, whole molecule
Conjugate: DyLight™ 405
Product Category: ChromPure® Purified Proteins from Normal Serums
RRID: AB_2337153