S R Group

This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)

Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.

Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.

The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.

The ELISA is based on the the immunoassay technique. The Microplate provided in this kit has been pre-coated with an Antibody specific to , make it to solid-phase antibody. Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for is added to each Microplate well and incubated, so the antibody-antigen-Enzyme labeled antibody complex is formed. Following a wash to remove any unbound reagent, then the TMB substrate solution is added to each well. Only those wells that contain and HRP-conjugated antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wave length of 450 nm. The qualitative determination of is determined by comparing with the CUTOFF value.

The ELISA is based on the the qualitative immunoassay technique. The Microplate provided in this kit has been pre-coated with an Antibody specific to anti-neuroblastoma-antibody,NB-Ab, make it to solid-phase antibody. Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for anti-neuroblastoma-antibody,NB-Ab is added to each Microplate well and incubated, so the antibody-antigen-Enzyme labeled antibody complex is formed. Following a wash to remove any unbound reagent, then the TMB substrate solution is added to each well. Only those wells that contain anti-neuroblastoma-antibody,NB-Ab and HRP-conjugated anti-neuroblastoma-antibody,NB-Ab antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wave length of 450 nm. The qualitative determination of anti-neuroblastoma-antibody,NB-Ab is determined by comparing with the CUTOFF value.

The ELISA is based on the the qualitative immunoassay technique. The Microplate provided in this kit has been pre-coated with an Antibody specific to anti-respiory syncytial virus (RSV) antibody (IgM), make it to solid-phase antibody. Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for anti-respiory syncytial virus (RSV) antibody (IgM) is added to each Microplate well and incubated, so the antibody-antigen-Enzyme labeled antibody complex is formed. Following a wash to remove any unbound reagent, then the TMB substrate solution is added to each well. Only those wells that contain anti-respiory syncytial virus (RSV) antibody (IgM) and HRP-conjugated anti-respiory syncytial virus (RSV) antibody (IgM) antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wave length of 450 nm. The qualitative determination of anti-respiory syncytial virus (RSV) antibody (IgM) is determined by comparing with the CUTOFF value.

This ELISA kit uses quantitative-ELISA as the method. The Micro-elisa strip plate provided in this kit has been pre-coated with an antibody specific to B-Cell Activating Factor (BAFF). Standards or samples are added to the appropriate Micro-elisa strip plate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for B-Cell Activating Factor (BAFF) is added to each Micro-elisa strip plate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain B-Cell Activating Factor (BAFF) and HRP conjugated B-Cell Activating Factor (BAFF) antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of B-Cell Activating Factor (BAFF). You can calculate the concentration of B-Cell Activating Factor (BAFF) in the samples by comparing the OD of the samples to the standard curve.

This assay uses the quantitative enzyme immunoassay method. The provided microtiter plate in the kit is pre-coated with BA (Butyric Acid). Standards or samples are added to the appropriate wells of the microtiter plate, along with a biotin-conjugated antibody specific to BA (Butyric Acid) Then, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Following this, TMB substrate solution is added. The enzyme-substrate reaction is stopped by adding a sulfuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BA (Butyric Acid) in the samples is determined by comparing the optical density (OD) of the samples to the standard curve.